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HELP: proteins identification questions



I analyze 2D-GEL protein spots by mass spectrometry (LC-MS/MS) and I have
some questions for you.

1) Do you know what are the characteristic that the scientific journals ask
in order to accept the identification of a 2D-GEL spot analyzed using
LC-MS/MS?

2) Is it sufficient to specify the protein molecular mass, Isoelectric
point, Mascot statistical Score, and number of identified peptides?

3) Is it necessary to reach a % of aminoacidic coverage or is it sufficient
to have a good agreement among the parameter specified in the previous
question (question 2)?

4) If yes what is this % coverage?

Please reply me both on the newsgroup and at my E-MAIL address:

[EMAIL PROTECTED]

I would like to thank you very much for your help.

Sincerely yours

Simone







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