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I want to design a "simple" spectrophotometer in order to obtain the difference in concentration between two samples. It should have the following features: -A light source (laser) that cross a sample, connected to a detector. -A second laser that cross a second sample, connected to a second detector. -Software to obtain the substract of the two signals, in order to convert in digital or analogical signal. My idea is that, if the samples contain only water and a same substance in different concentration, the difference in intensity between the two detectors will be equivalent to the difference in concentration. My question is: -Can this apparatus work using a detector of intensity, or maybe is needed separation in wavelengths? -What kind of detector and light I need, to reach a reasonable sensitivity? Thank you!
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