Re: Immunohisto problem

"Zbigniew Rudzki" <[EMAIL PROTECTED]> wrote in message news:<[EMAIL PROTECTED]>...
> Does anyone know the solution of the "strong edge, weak/negative center"
> problem we have with some routinely processed immuno slides (from paraffin
> blocks)?
> 
> What we get on the edge is a _real_ staining, i.e. the cells that should
> stain do stain, and these that should not do not.  The problems seems to
> affect more severly some antibodies (like DAKO LCA or CD20).  People from
> the company know about it (it is not only ours), but offer no reasonable
> solution.
> 
> Z. Rudzki
> staff pathologist
> Jagiellonian Univ., Poland

Your problem may be related to fixation and penetration of the
fixative. If the tissue sample is too large, the outside will be fixed
correctly, while the inside is not.

Frank