Re: Immunohisto problem

> Yes, we tried even in increased concentration, and much longer than
> recommended by standard protocols -- sometimes the improves the situation a
> little bit, usually not at all.  Extensive exosure to detergents requires
> also usually prolongation of the incubation with primary antibodies.
> 
> Z. Rudzki

pH of solution can greatly affect surfactants to the point of
neutralizing them. If the pH is alkaline, then that´s not the problem.