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Lithium Sunset napisał(a) w wiadomości: ... >Are you using a surfactant additive (eg TWEEN) in your diluent? It helps >a more even distribution of reagent/ antibodies during incubation. Yes, we tried even in increased concentration, and much longer than recommended by standard protocols -- sometimes the improves the situation a little bit, usually not at all. Extensive exosure to detergents requires also usually prolongation of the incubation with primary antibodies. Z. Rudzki
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