Re: Immunohisto problem

Are you using a surfactant additive (eg TWEEN) in your diluent? It helps 
a more even distribution of reagent/ antibodies during incubation.

Zbigniew Rudzki wrote:
> Does anyone know the solution of the "strong edge, weak/negative center"
> problem we have with some routinely processed immuno slides (from paraffin
> blocks)?
>
> What we get on the edge is a _real_ staining, i.e. the cells that should
> stain do stain, and these that should not do not.  The problems seems to
> affect more severly some antibodies (like DAKO LCA or CD20).  People from
> the company know about it (it is not only ours), but offer no reasonable
> solution.
>
> Z. Rudzki
> staff pathologist
> Jagiellonian Univ., Poland