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DNA bits joined together to make organism



        Next is to use a computer to predict the dna of dinosaur (from
the related birds dna) and bring back T rex

Craig Venter has artificially constructed the genetic blueprint of a
virus from scratch using bits of DNA. 

The virus, known as Phi-X174 (Phi-X), usually infects bacteria. It was
the first organism to have its genetic code read. Its genome consists
of 5386 segments of DNA arranged in a small circle. 

Previously, other researchers have synthesised the poliovirus genome
using enzymes that naturally occur in cells. 

However, that effort took years to achieve and produced viruses with
defects in their synthesised genetic code. 

 
Genome pioneer Craig Venter led the team 
In an effort to improve the speed and accuracy of this genomic
synthesis, Craig Venter and colleagues from the Institute for
Biological Energy Alternatives, have adapted a frequently used
technique - the polymerase chain reaction (PCR) into one called the
polymerase cycle assembly (PCA) - to reconstruct the genome of Phi-X. 

PCA is a technique that produces double-stranded copies of gene
sequences, ideal for assembling a genome. 

Works just like the 'natural' virus 

The researchers assembled several sections of the Phi-X genome from
oligonucleotides - small pieces of single-stranded DNA. 

After connecting these sections in an overlapping pattern, they used
PCA to completely construct the double-stranded viral genome. 

The entire process, which took 14 days, created viral DNA identical to
the known genetic code. 

The synthetic bacteriophage behaved just like the 'natural' one. It
had the ability to infect and kill bacterial cells and is
indistinguishable from its counterpart. 

The scientists say that although this technique is limited to simple
organisms, the ability to quickly and accurately synthesise long
segments of DNA could help them understand the function of particular
genes, and may be a stepping stone to manipulating more complex
organisms. 



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