Re: Fungi Isolation

"John Gentile" <[EMAIL PROTECTED]> wrote in message
news:[EMAIL PROTECTED]

> I've seen success with that kind of thinking. Our Mycology guy used to
> streak out a blood Mueller Hinton plate with the contaminated specimen and
> drop antibiotic disks - depending on the Gram stain. He usually was able
to
> pick off a pure fungal colony in the zone around one of the disks.
>
> Another idea is to be very careful and tease a tiny piece of the aerial
> hyphae and plant that to your selective media.

I've had success with the latter technique; an alternative is to grind up
some mycelium in some sterile water, maybe with tween, do a dilution series
and plate that out. At some point there ought to be single colonies of
bacterium and fungus- providing, of course, that each can grow without the
other.