Re: Fungi Isolation

Colin Davidson wrote:
> "Monica Enamorado" <[EMAIL PROTECTED]> wrote in message
> news:[EMAIL PROTECTED]
>
> > I'm working on fungi isolation for bioactive metabolites search,
> > nowadays I'm on the isolation stage.
> >
> > I have four samples of different fungi, these samples are polluted
> > with bacteria although the cultivation has a concentration of
> > cephotaxime of 5 micrograms/ml.
> >
> > I have checked for many possible sources of pollution, I have improved
> > the manipulation procedures but the pollution still persists.
> >
> > I would like to know about sterile procedures or antibiotics to
> > eliminate the bacteria from these samples. I would appreciate any
> > suggestions and references (papers, books, web sites) on this topic.
>
>
>
> Hmm. You're already using a fairly broad spectrum antibiotic, but you seem
> not to be getting rig of your contaminant that way. I'd try either upping
> the concentration of antibiotic or using a different antibiotic; you might
> just have something that isn't particularly susceptible to this antibiotic.
> I might be tempted to streak out the bacterium, Gram stain it and pick an
> appropriate antibiotic based on that.

Yeah, I agree. I'd streak out, stain a bacterial colony, and try some 
susceptibility tests on it.

Monica: Do you have access to an antibiotic disc dispenser? That will 
make it easier.

Scott.