Re: Fungi Isolation

"Monica Enamorado" <[EMAIL PROTECTED]> wrote in message
news:[EMAIL PROTECTED]
> I'm working on fungi isolation for bioactive metabolites search,
> nowadays I'm on the isolation stage.
>
> I have four samples of different fungi, these samples are polluted
> with bacteria although the cultivation has a concentration of
> cephotaxime of 5 micrograms/ml.
>
> I have checked for many possible sources of pollution, I have improved
> the manipulation procedures but the pollution still persists.
>
> I would like to know about sterile procedures or antibiotics to
> eliminate the bacteria from these samples. I would appreciate any
> suggestions and references (papers, books, web sites) on this topic.
>
> Thanks in advance,

You could try heat treatment. This is more likely to be successful if the
fungi are sporulating. Suspend some of the fungal tissue or spores in a
sterile liquid (saline or water) and heat to around 60 degrees C for 5 -15
minutes. That will kill most bacteria and should not injure the fungal
spores too much. Cool the suspension and plate out on a suitable medium (eg
Sabouraud agar).

GS