Re: Fungi Isolation

"Monica Enamorado" <[EMAIL PROTECTED]> wrote in message
news:[EMAIL PROTECTED]
> I'm working on fungi isolation for bioactive metabolites search,
> nowadays I'm on the isolation stage.
>
> I have four samples of different fungi, these samples are polluted
> with bacteria although the cultivation has a concentration of
> cephotaxime of 5 micrograms/ml.
>
> I have checked for many possible sources of pollution, I have improved
> the manipulation procedures but the pollution still persists.
>
> I would like to know about sterile procedures or antibiotics to
> eliminate the bacteria from these samples. I would appreciate any
> suggestions and references (papers, books, web sites) on this topic.


Hmm. You're already using a fairly broad spectrum antibiotic, but you seem
not to be getting rig of your contaminant that way. I'd try either upping
the concentration of antibiotic or using a different antibiotic; you might
just have something that isn't particularly susceptible to this antibiotic.
I might be tempted to streak out the bacterium, Gram stain it and pick an
appropriate antibiotic based on that.