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I'm working on fungi isolation for bioactive metabolites search, nowadays I'm on the isolation stage. I have four samples of different fungi, these samples are polluted with bacteria although the cultivation has a concentration of cephotaxime of 5 micrograms/ml. I have checked for many possible sources of pollution, I have improved the manipulation procedures but the pollution still persists. I would like to know about sterile procedures or antibiotics to eliminate the bacteria from these samples. I would appreciate any suggestions and references (papers, books, web sites) on this topic. Thanks in advance, Dr. Monica Enamorado Institute of Biotechnology National University of San Juan, Argentina
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