Re: uneven staining

Lithium Sunset schreef:
> 
> Is the chamber completely flat. Check for even a minor slope.
> 
> Peter Ashby wrote:
> > In article <[EMAIL PROTECTED]>,
> >  [EMAIL PROTECTED] (Shiri) wrote:
> >
> >
> >>I am getting un-even staining on 4 chamber slides. Portions of the
> >>chamber get stained beutifully, while others don't get stained at all.
> >>Any advise?
> >>I am staining cells in culture, that I fix with 4% PFA, to get a
> >>membrane-bound protein staining.
> >
> >
> > Are any areas of the slides drying out too much during processing?
> >
> > Peter
> >

Notice how the fluid tends to creep up at the edges of the chamber. If
the amount of fluid is small, parts of the center might easily become
dry.

Henk