Re: uneven staining

Is the chamber completely flat. Check for even a minor slope.

Peter Ashby wrote:
> In article <[EMAIL PROTECTED]>,
>  [EMAIL PROTECTED] (Shiri) wrote:
>
>
> > I am getting un-even staining on 4 chamber slides. Portions of the
> > chamber get stained beutifully, while others don't get stained at all.
> > Any advise?
> > I am staining cells in culture, that I fix with 4% PFA, to get a
> > membrane-bound protein staining.
>
>
> Are any areas of the slides drying out too much during processing? 
>
> Peter