Re: uneven staining

In article <[EMAIL PROTECTED]>,
 [EMAIL PROTECTED] (Shiri) wrote:

> I am getting un-even staining on 4 chamber slides. Portions of the
> chamber get stained beutifully, while others don't get stained at all.
> Any advise?
> I am staining cells in culture, that I fix with 4% PFA, to get a
> membrane-bound protein staining.

Are any areas of the slides drying out too much during processing? 

Peter

-- 
Peter Ashby
School of Life Sciences, University of Dundee, Scotland
To assume that I speak for the University of Dundee is to be deluded.
Reverse the Spam and remove to email me.