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Gene Quantification Newsletter December 2003 New features on the Gene-Quantification web page: Influence of zinc deficiency on the mRNA expression of zinc transporters in adult rats. http://www.wzw.tum.de/gene-quantification/zinc.html The mRNA expression of the members of the IGF-system in bovine corpus luteum during induced luteolysis Abundance of message for insulin-like growth factors-I and -II and for receptors for growth hormone, insulin-like growth factors-I and -II, and insulin in the intestine and liver of pre- and full-term calves. Effects of dexamethasone and colostrum intake on the somatotropic axis in neonatal calves. The mRNA expression of the members of the IGF-system in bovine corpus luteum during induced luteolysis. http://www.wzw.tum.de/gene-quantification/endo.html#igfs Expression and localisation of oestrogen and progesterone receptors in the bovine mammary gland during development, function and involution. The gastrointestinal tract as target of steroid hormone action: Quantification of steroid receptor mRNA expression (AR, ER-alpha, ER-beta and PR) in 10 bovine gastrointestinal tract compartments by kinetic RT-PCR. http://www.wzw.tum.de/gene-quantification/endo.html#erab Detection and quantification of mRNA-expression of alpha- and beta-adrenergic receptor subtypes in the mammary gland of dairy cows. Milking characteristics and their relation to adrenergic receptor mRNA expression and ligand binding in the mammary gland of dairy cows. http://www.wzw.tum.de/gene-quantification/endo.html#adrenerg New Section BioInformatics in real-time PCR http://www.wzw.tum.de/gene-quantification/bioinformatics.shtml Determination of real-time PCR efficiency http://www.wzw.tum.de/gene-quantification/efficiency.html PCR Efficiency estimation via "experimental four parametric logistic model fit" (including all fluorescence data points, Tichopad et al. 2003 - Standardized determination of real-time PCR efficiency from a single reaction set-up.) http://www.wzw.tum.de/gene-quantification/efficiency.html#e-fluoro-sig-3 Erratum of Figure 2 Tichopad et al. 2003 - http://www.wzw.tum.de/gene-quantification/efficiency.html#correction Improving quantitative real-time RT-PCR reproducibility by boosting primer-linked amplification efficiency http://www.wzw.tum.de/gene-quantification/efficiency.html#e-fluoro-sig-2 KOD (kinetic outlier detection) in real-time PCR (Tzachi Bar et al., 2003) http://www.wzw.tum.de/gene-quantification/efficiency.html#kod ######################################### Further, a lot of NEW real-time PCR workshops http://www.wzw.tum.de/gene-quantification/main.shtml#work NEW PUBLICATIONS about kinetic PCR http://www.wzw.tum.de/gene-quantification/real-time.html and http://www.wzw.tum.de/gene-quantification/literature.html Please send the NEWSLETTER and the home page links to further scientists and friends who are interested in real-time PCR, new quantification strategies, relative or absolute gene quantification using kinetic PCR. best regards Michael Pfaffl ######################################## First International qPCR Symposium & Application Workshop Transcriptomics, Clinical Diagnostics & Gene Quantification3rd - 6th March, 2004 in Freising-Weihenstephan, Germany http://www.wzw.tum.de/gene-quantification/qpcr2004/index.shtml Up to now around 135 Abstracts submitted from 27 Nations worldwide http://www.wzw.tum.de/gene-quantification/qpcr2004/main-2004.shtml#news The Symposium and the Application Workshop presents unbiased international lectures & posters about quantification strategies, normalisation methods & new algorithms for exact nucleic acid quantification in the broad range of qPCR applications: Transcriptomics, Clinical Diagnostics, Food Hygiene & GMO, Expression profiling, Microbiology, Pharmacogenomics, Forensic, Nutrigenomics, Molecular Endocrinology, cDNA Array Verification, Theoretical qPCR, Pre-analytical Steps & RT, Isolation of Nucleic Acids.http://www.wzw.tum.de/gene-quantification/qpcr2004/qpcr2004-workshop.html More than 40 internationally renown speakers will be participating in a lively and exciting programme enabling the valuable exchange of information in the qPCR field. As a special guest we can announce Prof. Carl Wittwer from the Institute of pathology at the University of Utah School of Medicine. He is an associate editor of Clinical Chemistry, and best known for developing techniques in rapid-cycle PCR and as the inventor of the LightCycler. http://www.wzw.tum.de/gene-quantification/qpcr2004/main-2004.shtml#speaker Parallel to the Symposium an Industrial Exhibition will take place along with the congress, where an intensive conversation with numerous companies is possible (real-time PCR Cyclers, Kit Producers, Nucleic Acid Purification Systems, Enzyme Producers, Plastic ware Suppliers, etc.). 23 companies agreed to participate at the Industrial Exhibition. http://www.wzw.tum.de/gene-quantification/qpcr2004/companies.htmlThe organisation team thank all Sponsors of the Symposium: http://www.wzw.tum.de/gene-quantification/qpcr2004/qpcr2004-sponsors.html Platinum Sponsor – Roche Diagnostics Gold Sponsor – BioRad Gold Sponsor – MJ Research Silver Sponsor – ABI Applied Biosystems Silver Sponsor – Invitrogen Silver Sponsor – Qiagen Silver Sponsor – Stratagene Symposium organisation & registration: Registration Form (PDF) http://www.wzw.tum.de/gene-quantification/qpcr2004/registration.pdf Registration Form (DOC) http://www.wzw.tum.de/gene-quantification/qpcr2004/registration.doc
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