Transfection of Tick Cell Lines

Hi ..

I don't know if anyone in this list has experience with continuous tick
cells lines, but I thought that I would try anyway.

I am trying to find out if there are any described methods for the
expression of heterologous proteins in continuous tick cell lines. I would
like to express proteins from Crimean Congo Hemorrhagic Fever virus using
plasmid expression constructs. Can these cells be transfected using CaPO4,
lipid reagents, or electroporation? Will mammalian promoter system work in
these cells types like CMV, SV40, etc? Alternatively, are there any
recombinant virus expression systems available?

I hope someone out there can help or can point me in the correct direction.

Thanks
-- 
Louis Altamura
Ph.D. Candidate in Cell and Molecular Biology (Microbiology/Virology)

University of Pennsylvania
302B Johnson Pavilion
3610 Hamilton Walk
Philadelphia, PA 19104